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ScienCellGK093 Human Renal Mesangial Cell Biology
更新時間:2023-01-03   點擊次數(shù):599次

GK093 ScienCell Human Renal Mesangial Cell Biology qPCR Array KitHuman Renal Mesangial Cell Biology qPCR Array Kit

ScienCell 研究實驗室(ScienCell)是一家生物技術(shù)公司,其使命是實驗和治療用細胞和細胞相關(guān)產(chǎn)品的研究和發(fā)展。 ScienCell為研究界提供各種高質(zhì)量的正常的人類和動物細胞,細胞培養(yǎng)基和試劑,培養(yǎng)基添加因子,細胞來源的RNAcDNA和蛋白質(zhì)。ScienCell的科學家們以改善生活質(zhì)量,實現(xiàn)細胞的能力,打擊人類退化性疾病為目的研究和開發(fā)各種細胞治療策略。ScienCell總部設(shè)在美國加利福尼亞州圣地亞哥,公司成立于1999年。

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GK093 ScienCell Human Renal Mesangial Cell Biology qPCR Array KitHuman Renal Mesangial Cell Biology qPCR Array Kit,產(chǎn)品簡介:

產(chǎn)品品牌: ScienCell

產(chǎn)品貨號:GK093

產(chǎn)品名稱:GK093 ScienCell Human Renal Mesangial Cell Biology qPCR Array KitHuman Renal Mesangial Cell Biology qPCR Array Kit

產(chǎn)品規(guī)格:One 96-well plate, one 20ul reaction per well


GK093 ScienCell Human Renal Mesangial Cell Biology qPCR Array Kit,Human Renal Mesangial Cell Biology qPCR Array Kit 產(chǎn)品說明:

ScienCellGeneQuery™人腎系膜細胞生物學qPCR陣列試劑盒(GQH-RMB)旨在促進參與基本系膜細胞生物學和腎小球生理學和病理生理學功能的88個關(guān)鍵基因的基因表達譜分析。如何根據(jù)其功能對所包含的基因進行分組的簡要示例如下所示:  

腎系膜細胞標志物:ACTA1FN1,PDGFRBTNS1,ITGA8

系膜基質(zhì)組裝:SERPINB7、膠原蛋白 4/5、層粘連蛋白 α/β/γ、SDC2HSPG2、CSPG5、NID1、ITGA8

生長因子調(diào)節(jié): IGF1, PTN, MFGE8, GDF10, CTGF NGF, PSEN2, SH3KBP1, ADAM12 AFAP1L2, SHC3

血管生成: VCAM1 ANGPT2, HOPX MYH11, NRP2, HDAC9, GJA5, ADAMTS1, FHL2, PLN, CTGF

內(nèi)吞作用:CLTA, CLTB CLTC, AP2A1 AP2A2, AP2B1, AP2M1, AP2S1, SMAD2, SMAD3, ZFYVE9 TGFB 受體

跨膜轉(zhuǎn)運蛋白活性: KCNMB1, GJA5, CACNA2D1 ABCC9, P2RX1, KCNRG, TRPCs, KCNQ5, PIEZO2

與以下因素有關(guān)的基因

系膜增生性腎小球腎炎:SERPINB7、IL6、CD79ACCL2、FGF2EDN1、CCR5、GAS6、AXL

彌漫性系膜硬化癥:ACTN4TRPC6、INF2、WT1、PLCE1PAX2、ARHGDIALAMB2、LAMC1

GeneQuery qPCR 芯片試劑盒采用 96 孔板格式的 qPCR 就緒,每個孔包含一個引物組,可以特異性識別并有效擴增靶基因的 cDNA。精心設(shè)計的引物確保:(iqPCR分析中的優(yōu)良退火溫度為65°C(含2 mM Mg2+,不含DMSO;ii)除非另有說明,否則引物組可識別靶基因的所有已知轉(zhuǎn)錄本變體;iii)只有一個基因被擴增。每個引物組都已通過qPCR和熔解曲線分析和凝膠電泳進行了驗證。

ScienCell's GeneQuery™ Human Renal Mesangial Cell Biology qPCR Array Kit (GQH-RMB) is designed to facilitate gene expression profiling of 88 key genes involved in essential mesangial cell biology and functions involved in glomerular physiology and pathophysiology. Brief examples of how included genes may be grouped according to their functions are shown below:    

·         Renal mesangial cell markers: ACTA1, FN1, PDGFRB, TNS1, ITGA8

·         Mesangial matrix assembly: SERPINB7, Collagen type 4/5, Laminin α/β/γ, SDC2, HSPG2, CSPG5, NID1, ITGA8

·         Growth factor modulation: IGF1, PTN, MFGE8, GDF10, CTGF, NGF, PSEN2, SH3KBP1, ADAM12, AFAP1L2, SHC3

·         Vasculogenesis: VCAM1, ANGPT2, HOPX, MYH11, NRP2, HDAC9, GJA5, ADAMTS1, FHL2, PLN, CTGF

·         Endocytosis: CLTA, CLTB, CLTC, AP2A1, AP2A2, AP2B1, AP2M1, AP2S1, SMAD2, SMAD3, ZFYVE9, TGFB receptors

·         Transmembrane transporter activity: KCNMB1, GJA5, CACNA2D1, ABCC9, P2RX1, KCNRG, TRPCs, KCNQ5, PIEZO2

·         Genes implicated in

o    Mesangial proliferative glomerulonephritis: SERPINB7, IL6, CD79A, CCL2, FGF2, EDN1, CCR5, GAS6, AXL

o    Diffuse mesangial sclerosis: ACTN4, TRPC6, INF2, WT1, PLCE1, PAX2, ARHGDIA, LAMB2, LAMC1

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.

基因查詢™人腎系膜細胞生物學qPCR陣列板布局*8 個粗體和斜體控件)


1

2

3

4

5

6

7

8

9

10

11

12

A

ABCC9

AP2A1

CCL2

COL5A1

FHL2

IGF1

LAMA2

MFGE8

PDGFRB

SFRP2

TGFBR3

β-actin

B

ACTA1

AP2A2

CCR5

COL5A2

FN1

IL6

LAMA3

MYH11

PIEZO2

SH3KBP1

TNS1

GAPDH

C

ACTN4

AP2B1

CD79A

COL5A3

GAS6

INF2

LAMA4

NGF

PLCE1

SHC3

TRPC1

LDHA

D

ADAM12

AP2M1

CLTA

CSPG5

GDF10

ITGA8

LAMA5

NID1

PLN

SMAD1

TRPC4

NONO

E

ADAMTS1

AP2S1

CLTB

CTGF

GJA5

KCNMB1

LAMB1

NID2

PSEN2

SMAD2

TRPC6

PPIH

F

AFAP1L2

ARHGDIA

CLTC

EDN1

HDAC9

KCNQ5

LAMB2

NRP2

PTN

SMAD3

VCAM1

GDC

G

AGTR1

AXL

COL4A1

ETS1

HOPX

KCNRG

LAMC1

P2RX1

SDC2

TGFBR1

WT1

PPC

H

ANGPT2

CACNA2D1

COL4A2

FGF2

HSPG2

LAMA1

MECOM

PAX2

SERPINB7

TGFBR2

ZFYVE9

NTC

提示:基因選擇可能會根據(jù)新的研究和開發(fā)進行更新

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