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ScienCellGK081人類周細胞生物學(xué)qPCR檢測試劑盒
更新時間:2023-01-03   點擊次數(shù):498次

GK081 ScienCell 人類周細胞生物學(xué)qPCR檢測試劑盒,Human Pericyte Biology qPCR Array Kit

ScienCell 研究實驗室(ScienCell)是一家生物技術(shù)公司,其使命是實驗和治療用細胞和細胞相關(guān)產(chǎn)品的研究和發(fā)展。 ScienCell為研究界提供各種高質(zhì)量的正常的人類和動物細胞,細胞培養(yǎng)基和試劑,培養(yǎng)基添加因子,細胞來源的RNAcDNA和蛋白質(zhì)。ScienCell的科學(xué)家們以改善生活質(zhì)量,實現(xiàn)細胞的能力,打擊人類退化性疾病為目的研究和開發(fā)各種細胞治療策略。ScienCell總部設(shè)在美國加利福尼亞州圣地亞哥,公司成立于1999年。

北京諾博萊德作為ScienCell品牌的經(jīng)銷商,為客戶提供,正規(guī)進口,貨期穩(wěn)定,折扣好的服務(wù)標準。

GK081 ScienCell人類周細胞生物學(xué)qPCR檢測試劑盒,Human Pericyte Biology qPCR Array Kit,產(chǎn)品簡介:

產(chǎn)品品牌: ScienCell

產(chǎn)品貨號:GK081

產(chǎn)品名稱:GK081 ScienCell人類周細胞生物學(xué)qPCR檢測試劑盒,Human Pericyte Biology qPCR Array Kit

產(chǎn)品規(guī)格:One 96-well plate, one 20ul reaction per well


GK081 ScienCell人類周細胞生物學(xué)qPCR檢測試劑盒,Human Pericyte Biology qPCR Array Kit, 產(chǎn)品說明:

ScienCell GeneQuery™ 人類周細胞生物學(xué) qPCR 陣列試劑盒 (GQH-PER) 分析了血管生成和免疫過程中參與周細胞功能的 88 個關(guān)鍵基因。 在脈管系統(tǒng)中,周細胞在小血管上充當(dāng)壁細胞,它們有助于維持血腦屏障(BBB),這是一種bukehuoque的穩(wěn)態(tài)大腦功能。 周細胞在免疫反應(yīng)中發(fā)揮作用時也具有巨噬細胞樣特性。 周細胞功能缺陷可導(dǎo)致BBB分解和異常脈管系統(tǒng)形成。 以下是如何根據(jù)其在周細胞生物學(xué)中的功能對所包含的基因進行分組的簡要示例:

血管生成:VEGFA,HIF1AJAG1,CTGF,ANGPT2

BBB 維護: ABCC9, VCAM1, OCLN, ICAM1, DLK1

炎癥: RIPK2, TLR3, CD74, CXCL9, APOL3

清除: FCGR2A, HLA-E, ITGAM C3, CD36

標記: CSPG4 NG2), PDGFRB MCAM, RGS5 CD248

GeneQuery qPCR 芯片試劑盒采用 96 孔板格式的 qPCR 就緒,每個孔包含一個引物組,可識別并有效擴增特定靶基因的 cDNA。精心設(shè)計的引物確保:(iqPCR分析中的優(yōu)良退火溫度為65°C(含2 mM Mg2+且不含DMSO;ii)除非另有說明,否則引物集可識別靶基因的所有已知轉(zhuǎn)錄本變體;iii)只有一個基因被擴增。每個引物組都已通過qPCR和熔解曲線分析和凝膠電泳進行了驗證。

ScienCell's GeneQuery™ Human Pericyte Biology qPCR Array Kit (GQH-PER) profiles 88 key genes involved in pericyte function during angiogenesis and in immunity.  In vasculature, pericytes function as mural cells on small blood vessels and they help maintain the blood-brain barrier (BBB), an integral homeostatic brain function.  Pericytes also possess macrophage-like properties when functioning in the immune response.  Defects in pericyte function can result in BBB breakdown and aberrant vasculature formation.  Below are brief examples of how included genes may be grouped according to their function in pericyte biology:

·         Angiogenesis: VEGFA, HIF1A, JAG1, CTGF, ANGPT2

·         BBB maintenance: ABCC9, VCAM1, OCLN, ICAM1, DLK1

·         Inflammation: RIPK2, TLR3, CD74, CXCL9, APOL3

·         Scavenging: FCGR2A, HLA-E, ITGAM, C3, CD36

·         Markers: CSPG4 (NG2), PDGFRB, MCAM, RGS5, CD248

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.

GeneQuery™ 人周細胞生物學(xué) qPCR 陣列板布局*8 個粗體和斜體對照)


1

2

3

4

5

6

7

8

9

10

11

12

A

ABCC9

APOL3

CD4

CSPG4

CXCL9

EGFR

HIF1A

ITGAM

NOTCH1

PTGS2

TLR3

β-actin

B

ACTA2

ARNT2

CD40

CTGF

CXCR3

EPHA2

HLA-DMA

JAG1

NOTCH3

RET

TNC

GAPDH

C

ADM

C1R

CD47

CXCL1

DES

ERBB2

HLA-E

KCNJ8

NOX4

RGS5

TNFRSF10D

LDHA

D

AGTR1

C3

CD68

CXCL10

DLK1

FCAR

ICAM1

MCAM

OCLN

RIPK2

TNFRSF14

NONO

E

ANGPT1

CCL2

CD74

CXCL11

EDN1

FCGR1A

IL32

MMP2

PDGFRB

SOD2

TPM1

PPIH

F

ANGPT2

CCL8

CDH2

CXCL12

EDNRA

FCGR2A

IL6

MYLK

PECAM1

TGFB1

VCAM1

GDC

G

ANPEP

CD248

CREB5

CXCL2

EDNRB

GJB6

IRF1

NFKB1

PRKAA1

TGFB3

VEGFA

PPC

H

APOL2

CD36

CREBBP

CXCL8

EFNB2

HBEGF

ITGA5

NOS2

PSMB8

TGFBR1

VEGFC

NTC

提示:基因選擇可能會根據(jù)新的研究和開發(fā)進行更新

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