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ScienCellGK080人成骨分化qPCR檢測試劑盒
更新時間:2023-01-03   點擊次數(shù):469次

GK080 ScienCell 人成骨分化qPCR檢測試劑盒,GeneQuery Human Osteogenic Differentiation qPCR Array Kit

ScienCell 研究實驗室(ScienCell)是一家生物技術公司,其使命是實驗和治療用細胞和細胞相關產品的研究和發(fā)展。 ScienCell為研究界提供各種高質量的正常的人類和動物細胞,細胞培養(yǎng)基和試劑,培養(yǎng)基添加因子,細胞來源的RNA,cDNA和蛋白質。ScienCell的科學家們以改善生活質量,實現(xiàn)細胞的能力,打擊人類退化性疾病為目的研究和開發(fā)各種細胞治療策略。ScienCell總部設在美國加利福尼亞州圣地亞哥,公司成立于1999年。

北京諾博萊德作為ScienCell品牌的經銷商,為客戶提供,正規(guī)進口,貨期穩(wěn)定,折扣好的服務標準。

GK080 ScienCell人成骨分化qPCR檢測試劑盒,GeneQuery Human Osteogenic Differentiation qPCR Array Kit,產品簡介:

產品品牌: ScienCell

產品貨號:GK080

產品名稱:GK080 ScienCell人成骨分化qPCR檢測試劑盒,GeneQuery Human Osteogenic Differentiation qPCR Array Kit

產品規(guī)格:One 96-well plate, one 20ul reaction per well


GK080 ScienCell人成骨分化qPCR檢測試劑盒,GeneQuery Human Osteogenic Differentiation qPCR Array Kit 產品說明:

ScienCell GeneQuery™ 人類成骨分化 qPCR 陣列 (GQH-OST) 有助于對參與間充質干細胞分化為骨細胞、成骨細胞和破骨細胞的 88 個關鍵基因進行基因表達分析。 骨細胞最常見于成熟骨組織中,能夠長距離傳輸信號。 成骨細胞和破骨細胞是分別合成和分解骨骼的骨細胞。 如何根據(jù)其功能對所包含的基因進行分組的簡要示例如下所示:

骨細胞標志物:BGNFGF23,PDPN,HYOU1SOST

成骨細胞標志物:GNL3,CD44OMDMME,SCUBE3

破骨細胞標志物:CA2CALCR,CTSK,MMP9TNFRSF11A

RUNX2 抑制器: GLI3, HES1, STAT1, TWIST1, HAND2

BMP 信令:BMP2、BMP4SMAD4、SMAD5、SMAD9

GeneQuery qPCR 芯片試劑盒采用 96 孔板格式的 qPCR 就緒,每個孔包含一個引物組,可識別并有效擴增特定靶基因的 cDNA。精心設計的引物確保:(iqPCR分析中的優(yōu)良退火溫度為65°C(含2 mM Mg2+且不含DMSO;ii)除非另有說明,否則引物集可識別靶基因的所有已知轉錄本變體;iii)只有一個基因被擴增。每個引物組都已通過qPCR和熔解曲線分析和凝膠電泳進行了驗證。

ScienCell's GeneQuery Human Osteogenic Differentiation qPCR Array (GQH-OST) facilitates gene expression profiling of 88 key genes involved in the differentiation of mesenchymal stem cells into osteocytes, osteoblasts, and osteoclasts.  Osteocytes are most commonly found in mature bone tissue and capable of signal transmission over long distances.  Osteoblasts and osteoclasts are bone cells that synthesize and break down bone, respectively.  Brief examples of how included genes may be grouped according to their function are shown below:

·         Osteocyte marker: BGN, FGF23, PDPN, HYOU1, SOST

·         Osteoblast marker: GNL3, CD44, OMD, MME, SCUBE3

·         Osteoclast marker: CA2, CALCR, CTSK, MMP9, TNFRSF11A

·         RUNX2 suppressor: GLI3, HES1, STAT1, TWIST1, HAND2

·         BMP signaling: BMP2, BMP4, SMAD4, SMAD5, SMAD9

GeneQuery qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.

基因查詢™人類成骨分化 qPCR 陣列板布局*8 個粗體和斜體對照)


1

2

3

4

5

6

7

8

9

10

11

12

A

ALCAM

CA2

CSNK2A1

FGF2

FZD9

HYOU1

MAF

NKX3-2

RBPJ

SMO

TCF7

β-actin

B

ALPL

CALCR

CTNNB1

FGF23

GLI2

IBSP

MCAM

NOTCH1

RUNX2

SOST

TGFB1

GAPDH

C

ATF4

CAPG

CTSK

FGFR1

GLI3

IGF1R

MEPE

NOTCH2

SATB2

SOX9

TNFRSF11A

LDHA

D

BCAP31

CD44

DCN

FGFR2

GNL3

IGFBP3

MME

OMD

SCUBE3

SP7

TNFSF11

NONO

E

BGN

CD59

DMP1

FGFR3

HAND2

ITGB1

MMP14

PCOLCE

SMAD1

SPARC

TPO

PPIH

F

BMP2

COL1A1

DSTN

FN1

HES1

LEF1

MMP9

PDPN

SMAD4

SPP1

TWIST1

GDC

G

BMP4

COL1A2

ENG

FOSB

HEY1

LRP5

MSX2

PHEX

SMAD5

STAT1

TXLNG

PPC

H

BMPR1A

COL2A1

FGF18

FOSL1

HIVEP3

LRP6

NFATC1

PRKCD

SMAD9

TAZ

WNT5A

NTC

提示:基因選擇可能會根據(jù)新的研究和開發(fā)進行更新

ScienCell基因表達分析產品訂購信息:

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GK010   人類黑素細胞發(fā)育和色素沉著qPCR檢測試劑盒,Human Melanocyte Development & Pigmentation qPCR Array Kit

GK011   人類黑素細胞生物學qPCR檢測試劑盒,Human Melanocyte Cell Biology qPCR Array Kit

GK013   人類星型膠質細胞轉錄因子qPCR檢測試劑盒,Human Astrocyte Transcription Factors qPCR Array Kit

GK017   人血管生成qPCR檢測試劑盒,Human Angiogenesis qPCR Array Kit

GK018   人內皮細胞表型異質性qPCR檢測試劑盒,Human Endothelial Cell Phenotypic Heterogeneity qPCR Array Kit

GK019   人內皮細胞生物學qPCR檢測試劑盒,Human Endothelial Cell Biology qPCR Array Kit

GK020   B細胞受體信號通路qPCR檢測試劑盒,Human B Cell Receptor Signaling Pathway qPCR Array Kit

GK021   人類T細胞受體信號通路qPCR檢測試劑盒,Human T Cell Receptor Signaling Pathway qPCR Array Kit

GK022   人內吞作用qPCR檢測試劑盒,Human Endocytosis qPCR Array Kit

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GK025   人類Notch信號通路qPCR檢測試劑盒,uman Notch Signaling Pathway qPCR Array Kit

GK026   人類Wnt信號通路qPCR檢測試劑盒,Human Wnt Signaling Pathway qPCR Array Kit

GK027   人類非經典Wnt信號通路qPCR檢測試劑盒,Human Noncanonical Wnt Signaling Pathways qPCR Array Kit

GK028   人類Wnt信號靶基因qPCR檢測試劑盒,Human Wnt Signaling Target Genes qPCR Array Kit

GK029   Human Tie2/Angpt信號qPCR檢測試劑盒,Human Tie2/Angpt Signaling qPCR Array Kit

GK034   人肺纖維化qPCR檢測試劑盒,Human Pulmonary Fibrosis qPCR Array Kit

GK035   人體缺氧反應qPCR檢測試劑盒,Human Hypoxia Response qPCR Array Kit

GK036   人類HIF1信號響應qPCR檢測試劑盒,Human HIF1 Signaling Response qPCR Array Kit

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GK073   人類小膠質細胞Sensome qPCR檢測試劑盒,Human Microglial Sensome qPCR Array Kit

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GK078   人類神經細胞生物學qPCR檢測試劑盒,Human Neuronal Cell Biology qPCR Array Kit

GK079   人類軟骨細胞生物學qPCR檢測試劑盒,Human Chondrocyte Biology qPCR Array Kit

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