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ScienCellGK021人類T細(xì)胞受體信號(hào)通路qPCR檢測(cè)試劑盒
更新時(shí)間:2023-01-03   點(diǎn)擊次數(shù):598次

GK021 ScienCell 人類T細(xì)胞受體信號(hào)通路qPCR檢測(cè)試劑盒,Human T Cell Receptor Signaling Pathway qPCR Array Kit

ScienCell 研究實(shí)驗(yàn)室(ScienCell)是一家生物技術(shù)公司,其使命是實(shí)驗(yàn)和治療用細(xì)胞和細(xì)胞相關(guān)產(chǎn)品的研究和發(fā)展。 ScienCell為研究界提供各種高質(zhì)量的正常的人類和動(dòng)物細(xì)胞,細(xì)胞培養(yǎng)基和試劑,培養(yǎng)基添加因子,細(xì)胞來源的RNA,cDNA和蛋白質(zhì)。ScienCell的科學(xué)家們以改善生活質(zhì)量,實(shí)現(xiàn)細(xì)胞的能力,打擊人類退化性疾病為目的研究和開發(fā)各種細(xì)胞治療策略。ScienCell總部設(shè)在美國(guó)加利福尼亞州圣地亞哥,公司成立于1999年。

北京諾博萊德作為ScienCell品牌的經(jīng)銷商,為客戶提供,正規(guī)進(jìn)口,貨期穩(wěn)定,折扣好的服務(wù)標(biāo)準(zhǔn)。

GK021 ScienCell人類T細(xì)胞受體信號(hào)通路qPCR檢測(cè)試劑盒,Human T Cell Receptor Signaling Pathway qPCR Array Kit,產(chǎn)品簡(jiǎn)介:

產(chǎn)品品牌; ScienCell

產(chǎn)品貨號(hào):GK021

產(chǎn)品名稱:GK021 ScienCell人類T細(xì)胞受體信號(hào)通路qPCR檢測(cè)試劑盒,Human T Cell Receptor Signaling Pathway qPCR Array Kit

產(chǎn)品規(guī)格:One 96-well plate, one 20ul reaction per well


GK021 ScienCell人類T細(xì)胞受體信號(hào)通路qPCR檢測(cè)試劑盒,Human T Cell Receptor Signaling Pathway qPCR Array Kit, 產(chǎn)品說明:

ScienCell GeneQuery™ 人 T 細(xì)胞受體信號(hào)通路 qPCR 陣列試劑盒 (GQH-TRS) 旨在促進(jìn)參與 T 細(xì)胞受體信號(hào)通路的 88 個(gè)關(guān)鍵基因的基因表達(dá)譜分析。如何根據(jù)其功能對(duì)所包含的基因進(jìn)行分組的簡(jiǎn)要示例如下所示:

T細(xì)胞受體復(fù)合物成分:CD3D,CD3E,CD3G

輔助受體:CD4CD8A、CD8B

輔助抑制劑:PDCD1、CTLA4

共刺激物:ICOSCD28

激酶:FYN,ZAP70

MAPK途徑:KRAS,HRAS,NRASRAF1,MAP2K1,MAPK1,MAPK3

PI3K/AKT 通路:PI3K、AKT1、AKT2

IKK/NFKB途徑:NFKB,DAG1,BCL10CARD11,MAP3K7,MALT1,CHUK,IKBKBIKBKG

GeneQuery qPCR 芯片試劑盒采用 96 孔板格式的 qPCR 就緒,每個(gè)孔包含一個(gè)引物組,可以特異性識(shí)別并有效擴(kuò)增靶基因的 cDNA。精心設(shè)計(jì)的引物確保:(iqPCR分析中的優(yōu)良退火溫度為65°C(含2 mM Mg2+,不含DMSO;ii)除非另有說明,否則引物組可識(shí)別靶基因的所有已知轉(zhuǎn)錄本變體;iii)只有一個(gè)基因被擴(kuò)增。每個(gè)引物組都已通過qPCR和熔解曲線分析和凝膠電泳進(jìn)行了驗(yàn)證。

ScienCell's GeneQuery Human T Cell Receptor Signaling Pathway qPCR Array Kit (GQH-TRS) is designed to facilitate gene expression profiling of 88 key genes involved in T cell receptor signaling pathway. Brief examples of how included genes may be grouped according to their functions are shown below:    

·         T cell receptor complex components: CD3D, CD3E, CD3G

·         Co-receptors: CD4, CD8A, CD8B

·         Co-inhibitors: PDCD1, CTLA4

·         Co-stimulators: ICOS, CD28

·         Kinases: FYN, ZAP70

·         MAPK pathway: KRAS, HRAS, NRAS, RAF1, MAP2K1, MAPK1, MAPK3

·         PI3K/AKT pathway: PI3Ks, AKT1, AKT2

·         IKK/NFKB pathway: NFKBs, DAG1, BCL10, CARD11, MAP3K7, MALT1, CHUK, IKBKB, IKBKG

GeneQuery qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.

基因查詢™人 T 細(xì)胞受體信號(hào)通路 qPCR 陣列板布局*8 個(gè)粗體和斜體控件)


1

2

3

4

5

6

7

8

9

10

11

12

A

AKT1

CD274

CD8A

DYRK1A

HRAS

MALT1

NCK1

NFKBIE

PDPK1

PPP3CB

RELA

β-actin

B

AKT2

CD28

CD8B

DYRK2

ICOS

MAP2K1

NCK2

NRAS

PIK3CA

PPP3R1

SOS1

GAPDH

C

BCL10

CD3D

CDC42

FOS

IKBKB

MAP2K7

NFATC1

NRON

PIK3CB

PRKCA

SOS2

LDHA

D

CALM1

CD3E

CDK4

FYN

IKBKG

MAP3K7

NFATC2

PAK1

PIK3R1

PRKCQ

TEC

NONO

E

CARD11

CD3G

CHUK

GRAP2

ITK

MAP3K8

NFATC3

PAK2

PIK3R2

PTCRA

TNF

PPIH

F

CBL

CD4

CTLA4

GRB2

JUN

MAPK1

NFKB1

PAK3

PIK3R3

PTPN6

VAV1

GDC

G

CBLB

CD80

DAG1

GSK3A

KRAS

MAPK3

NFKBIA

PAK4

PLCG1

RAF1

VAV2

PPC

H

CBLC

CD86

DLG1

GSK3B

LCP2

MAPK9

NFKBIB

PDCD1

PPP3CA

RASGRP1

ZAP70

NTC

提示:基因選擇可能會(huì)根據(jù)新的研究和開發(fā)進(jìn)行更新

ScienCell基因表達(dá)分析產(chǎn)品訂購(gòu)信息:

GK001   人類細(xì)胞凋亡qPCR檢測(cè)試劑盒,Human Apoptosis qPCR Array Kit

GK002   人類多能干細(xì)胞qPCR檢測(cè)試劑盒,Human Pluripotent Stem Cell qPCR Array Kit

GK003   人類細(xì)胞周期qPCR檢測(cè)試劑盒,Human Cell Cycle qPCR Array Kit

GK005   人上皮細(xì)胞生物學(xué)qPCR檢測(cè)試劑盒,Human Epithelial Cell Biology qPCR Array Kit

GK006   人類神經(jīng)發(fā)育和再生qPCR檢測(cè)試劑盒,Human Neural Development and Regeneration qPCR Array Kit

GK007   人類神經(jīng)可塑性qPCR檢測(cè)試劑盒,Human Neural Plasticity qPCR Array Kit

GK008   人類神經(jīng)傳遞和膜轉(zhuǎn)運(yùn)qPCR檢測(cè)試劑盒,Human Neural Transmission and Membrane Trafficking qPCR Array Kit

GK009   人類星型膠質(zhì)細(xì)胞生物學(xué)qPCR檢測(cè)試劑盒,Human Astrocyte Cell Biology qPCR Array Kit

GK010   人類黑素細(xì)胞發(fā)育和色素沉著qPCR檢測(cè)試劑盒,Human Melanocyte Development & Pigmentation qPCR Array Kit

GK011   人類黑素細(xì)胞生物學(xué)qPCR檢測(cè)試劑盒,Human Melanocyte Cell Biology qPCR Array Kit

GK013   人類星型膠質(zhì)細(xì)胞轉(zhuǎn)錄因子qPCR檢測(cè)試劑盒,Human Astrocyte Transcription Factors qPCR Array Kit

GK017   人血管生成qPCR檢測(cè)試劑盒,Human Angiogenesis qPCR Array Kit

GK018   人內(nèi)皮細(xì)胞表型異質(zhì)性qPCR檢測(cè)試劑盒,Human Endothelial Cell Phenotypic Heterogeneity qPCR Array Kit

GK019   人內(nèi)皮細(xì)胞生物學(xué)qPCR檢測(cè)試劑盒,Human Endothelial Cell Biology qPCR Array Kit

GK020   B細(xì)胞受體信號(hào)通路qPCR檢測(cè)試劑盒,Human B Cell Receptor Signaling Pathway qPCR Array Kit

GK021   人類T細(xì)胞受體信號(hào)通路qPCR檢測(cè)試劑盒,Human T Cell Receptor Signaling Pathway qPCR Array Kit

GK022   人內(nèi)吞作用qPCR檢測(cè)試劑盒,Human Endocytosis qPCR Array Kit






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