伯樂Bio-Rad1610734 10x Tris/Glycine Buffer for Western Blots and Native Gels,10倍Tris/甘氨酸緩沖液,用于Western blot和天然凝膠
伯樂Bio-Rad公司總部設(shè)在美國加里福尼亞州的Hercules,Bio-Rad在quanqiu擁有30多家全資子公司,服務(wù)于quanqiu超過85,000家生物技術(shù)公司、生物制藥公司、科學(xué)研究機(jī)構(gòu)和臨床實(shí)驗(yàn)室。Bio-Rad公司自1952年成立以來,在60多年中為臨床診斷和生命科學(xué)研究市場提供了guangfan的新型產(chǎn)品和服務(wù),始終居于科學(xué)發(fā)現(xiàn)的中心地位。
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伯樂Bio-Rad1610734 10x Tris/Glycine Buffer for Western Blots and Native Gels,10倍Tris/甘氨酸緩沖液,用于Western blot和天然凝膠,產(chǎn)品簡介:
產(chǎn)品品牌:伯樂Bio-Rad
產(chǎn)品名稱:10倍Tris/甘氨酸緩沖液,用于Western blot和天然凝膠
英文名稱:10x Tris/Glycine Buffer for Western Blots and Native Gels
產(chǎn)品貨號(hào):1610734
產(chǎn)品規(guī)格:1 L
伯樂Bio-Rad1610734 10x Tris/Glycine Buffer for Western Blots and Native Gels,10倍Tris/甘氨酸緩沖液,用于Western blot和天然凝膠,產(chǎn)品說明:
Pkg of 1, 1 L, 10x premixed electrophoresis buffer contains 25 mM Tris, 192 mM glycine, pH 8.3 following dilution to 1x with water
每包1,1 L,10倍預(yù)混電泳緩沖液含25 mM Tris,192 mM甘氨酸,用水稀釋至1x后pH值為8.3
預(yù)混合轉(zhuǎn)印緩沖液和試劑
有2種轉(zhuǎn)印緩沖液可供選擇: 10 x Tris/甘氨酸和10x Tris/CAPS。預(yù)混合封閉緩沖液有1xPBS/干酪素和1xTBS/干酪素兩種,可節(jié)省溶解干酪素所需的時(shí)間和精力。Bio Rad提供完整系列的緩沖液試劑,可根據(jù)您的特殊需要制備緩沖液。
預(yù)混緩沖液可用于多種蛋白質(zhì)和核酸電泳實(shí)驗(yàn)方案。 經(jīng)0.4 µm濾膜過濾的高質(zhì)量緩沖液可用于手灌或預(yù)制膠。
使用預(yù)混電泳緩沖液可縮短制備時(shí)間,同時(shí)可以避免可能發(fā)生的緩沖液濃度錯(cuò)誤。 此外,預(yù)混的電泳緩沖液可幫助您將電泳運(yùn)行標(biāo)準(zhǔn)化,因?yàn)樗鼈兌际峭ㄟ^ Bio-Rad 電泳分離純度試劑制成的,可通過質(zhì)量控制來獲得可重復(fù)的結(jié)果。 這些緩沖液經(jīng)過 0.4 µm 過濾,可確保獲得最高質(zhì)量。 沒有要稱量或過濾的試劑;只需用去離子的蒸餾水稀釋即可。
預(yù)混緩沖液適用于各種蛋白質(zhì)和核酸電泳實(shí)驗(yàn)方案,并且可用于手灌或預(yù)制膠。 5 L 的盒經(jīng)濟(jì)且方便使用;它們小巧緊湊且可堆疊,從而節(jié)省操作臺(tái)占用面積,并且它們?cè)谠O(shè)計(jì)上具有簡易倒口。
使用10x Tris/甘氨酸緩沖液作為western印跡的轉(zhuǎn)移緩沖液或作為天然蛋白質(zhì)凝膠電泳的流動(dòng)緩沖液。
用于SDS-PAGE凝膠的罐式或半干法印跡,通常添加20%甲醇
不含甲醇的天然凝膠罐吸墨劑
作為天然凝膠的運(yùn)行緩沖區(qū)
天然電泳的預(yù)制凝膠分類:
Precast Gel Type | Size | Gel Chemistry |
Mini-PROTEAN TGX | Mini | Laemmli-like, long shelf life, fast separation with high resolution |
Criterion TGX | Midi | |
Mini-PROTEAN TGX Stain-Free | Mini | Laemmli-like, long shelf life, fast separation with high resolution, unique trihalo compounds for rapid fluorescent protein detection |
Criterion TGX Stain-Free | Midi | |
Criterion Tris-HCl | Midi | Standard Laemmli |
Criterion Stain Free Tris-HCl | Midi | Standard Laemmli, unique trihalo compounds for rapid fluorescent protein detection |
Criterion XT Tris-Acetate | Midi | Discontinuous buffer ion fronts form moving boundaries to stack, and then separate proteins |
注:大多數(shù)蛋白質(zhì)具有酸性或稍堿性pI(~3–8),并且與SDS-PAGE一樣,在連接到電泳室的電源下運(yùn)行。如果更多的堿性蛋白質(zhì)(pl>8.5)被分離出來,改變電極的極性,因?yàn)樗鼈儙в袃粽姾伞?/p>
轉(zhuǎn)印緩沖液轉(zhuǎn)印指南
| 1x配方 | 應(yīng)用 |
印跡轉(zhuǎn)移緩沖液* |
|
|
10xTris甘氨酸 | 25 mM Tris, 192 mM甘氨酸,pH8.3 | Western雜交 |
10x TrisCAPS | 正極緩沖液: 60 mM Tris, 40 mM CAPS, 15% 甲醇,pH9.6 負(fù)極緩沖液: 60 mM Tris, 40 mM CAPS, 0.1% (w/v)SDS, pH9.6 | 不連續(xù)緩沖液,提高半干應(yīng)用中的轉(zhuǎn)移效率 |
印跡膜處理緩沖液 |
|
|
10x PBS | 10 mM磷酸鈉, 150 mMlvhuana,pH 7.4 | Western雜交膜洗滌液 |
10x TBS | 20 mM Tris, 500 mMlvhuana,pH 7.4 | Western雜交膜洗滌液 |
1 xPBS/1%于酪素 | 10 mM磷酸鈉, 150 mMlvhuana,1% (w/v)干酪素,pH7.4 | Western印跡膜封閉緩沖液 (干酪素封閉劑推薦用于所有應(yīng)用,包括生物素- 親和素復(fù)合體) |
1xTBS with1%干酪素 | 20 mM Tris, 500 mMlvhuana, 含有1% (w/v)干酪素,pH7.4 | Western印跡膜封閉緩沖液(干酪素封阻劑推薦用于所有應(yīng)用,包括生物素-親和素復(fù)合體) |
"緩沖液可用于所有凝膠類型和配方。
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PrecisionAb驗(yàn)證的免疫印跡抗體PrecisionAb Validated Western Blotting Antibodies
本機(jī)樣本緩沖區(qū)(1610738)Native Sample Buffer (1610738)
帶蓋標(biāo)準(zhǔn)染色/吸墨紙盤(3459921)Criterion Staining/Blotting Trays with lids (3459921)
10倍磷酸鹽緩沖鹽水(PBS)(1610780)10x Phosphate Buffered Saline (PBS) (1610780)
10倍Tris緩沖鹽水(TBS)(1706435)10x Tris Buffered Saline (TBS) (1706435)
1x磷酸鹽緩沖鹽水(PBS),含1%酪蛋白(1610783)1x Phosphate Buffered Saline (PBS) with 1% Casein (1610783)
1x Tris緩沖鹽水(TBS),含1%酪蛋白(1610782)1x Tris Buffered Saline (TBS) with 1% Casein (1610782)
吸墨劑級(jí)阻滯劑,脫脂奶粉(1706404)Blotting-Grade Blocker, nonfat dry milk (1706404)
伯樂Bio-Rad1610734 10x Tris/Glycine Buffer for Western Blots and Native Gels,10倍Tris/甘氨酸緩沖液,用于Western blot和天然凝膠,產(chǎn)品訂購信息:
Blot Transfer and Processing Buffers
伯樂Bio-Rad 1610734 10x Tris/Glycine, 1 L
伯樂Bio-Rad 1610771 10x Tris/Glycine, 5 L cube
伯樂Bio-Rad 1610778 10x Tris/CAPS, 1 L
伯樂Bio-Rad 1610780 10x Phosphate Buffered Saline, 1L
伯樂Bio-Rad 1706435 10x Tris Buffered Saline, 1 L
Detergents and Blocking Reagents
伯樂Bio-Rad 1706537 Gelatin, EIA grade, 200g
伯樂Bio-Rad 1706404 Blotting-Grade Blocker, nonfat dry milk, 300 g
伯樂Bio-Rad 1706531 Tween 20, EIA grade, 100 ml
伯樂Bio-Rad 1610781 10% (w/v) Tween 20, for easy pipetting, 1 L
伯樂Bio-Rad 1610418 SDS Solution, 20% (w/V), 1 L
伯樂Bio-Rad 1610783 1x Phosphate Buffered Saline with 1% Casein*, 1 l
伯樂Bio-Rad 1610782 1x Tris Buffered Saline with 1% Casein*, 1 L
Reagents
伯樂Bio-Rad 1610610 Dithiothreitol (DTT)*, 1 g
伯樂Bio-Rad 1610611 Dithiothreitol (DTT)*, 5g
伯樂Bio-Rad 1610729 EDTA, 500 g
伯樂Bio-Rad 1706537 Gelatin, EIA grade, 200 g
伯樂Bio-Rad 1610717 Glycine, 250 g
伯樂Bio-Rad 1610718 Glycine, 1 kg
伯樂Bio-Rad 1610710 2-Mercaptoethanol, 25 ml
伯樂Bio-Rad 1632101 Tributylphosphine (TBP), 200 mM, 0.6 m
伯樂Bio-Rad 1610713 Tricine, 500 g
伯樂Bio-Rad 1610716 Tris, 500 g
伯樂Bio-Rad 1610719 Tris, 1 kg
伯樂Bio-Rad 1610730 Urea, 250 g
儲(chǔ)存在2--8°C。
**在2--8°C下干燥儲(chǔ)存;在室溫下儲(chǔ)存其他試劑,干燥,遠(yuǎn)離陽光直射。危險(xiǎn)運(yùn)輸費(fèi)